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Hansi Flick's men, with a commanding 4-0 lead from the first leg, will be looking to progress to the semi-finals against either Inter Milan or Bayern Munich.
The squad list includes the likes of Lewandowski, De Jong, Pedri and Raphinha, while Balde misses out with a hamstring injury sustained during Barca's win over Leganes at the weekend. Olmo also returns to the squad after recovering from an adductor problem.
Barcelona's defensive line will feature Gerard Martin as a replacement for Balde, alongside Kounde, Araujo and Cubarsi. Szczesny is expected to start in goal, providing stability against Dortmund's counter-attacking threat.
Raphinha is just a goal or an assist away from beating Messi's record for the most goals in a single Champions League campaign for Barcelona. Borussia Dortmund will need a historic comeback to overturn their deficit, but Barcelona are wary of underestimating their opponents in front of a passionate German crowd.
