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With Loum Tchaouna, Fisayo Dele-Bashiru, and a stunning Pedro goal, Lazio defeated Ajax 3-1 on the road, securing their spot in the Europa League's next round. Up to their contentious 0-0 match against Ludogorets, where a blatant penalty was disallowed despite the VAR review, the Aquile had won every match in the competition. Due to security concerns, their supporters were not permitted in the Johan Cruijff ArenA, and Alessio Romagnoli and Matias Vecino suffered injuries. Owen Wijndal, Davy Klaassen, Sivert Mannsverk, Amourricho Van Axel-Dongen, Gaston Avila, and former Roma midfielder Benjamin Tahirovic were all suspended by Francesco Farioli's Ajax.
Taty Castellanos nearly jumped on a weak back ball from Youri Baas, but Bertrand Traoré curled wide, serving as only a warning for the first goal. To defeat Remko Pasveer one-on-one, Castellanos sent Tchaouna onto a through ball after Boulaye Dia pushed Kenneth Taylor into a misdirected pass near midfield.
